In the field of life science research, cell culture is a critical link for exploring the mysteries of cells and driving medical progress. Collagen, as an important "partner" in cell culture, plays an indispensable role.

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Collagen is widely present in connective tissues and the extracellular matrix of internal organs, with a large family of members. The common types include Type I, Type II, Type III, and Type IV. Among them,?Type I Collagen?is highly favored in in vitro cell culture due to its unique advantages. It is widely distributed in fibrous connective tissues such as skin, bones, and tendons. Not only does it act as a natural adhesive, but it also excels in promoting cell adhesion, maintaining cell functions, and enhancing cell morphological specificity.

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The traditional?2D cell culture?method involves growing cells on glass or plastic surfaces, where cells can only spread in a two-dimensional space. This method is not ideal for certain primary cells (e.g., nerve cells, stem cells), as these cells struggle to attach to conventional flat surfaces. In such cases,?collagen coating?is required for assistance.

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In contrast, the?3D solid gel?prepared with collagen forms a three-dimensional network structure, which is simply an "ideal home" for cell culture. It can highly simulate the in vivo tissue structure and microenvironment, meet the needs of complex interactions between cells, and create better conditions for cell growth and differentiation. Precisely because of this, 3D cell culture shows great potential in multiple research fields:

·?Tumor Research: It can construct a tumor microenvironment that is closer to the real in vivo state, narrowing the gap between 2D cell lines, animal models, and clinical research, thereby facilitating new breakthroughs in tumor research.

·?Pharmaceutical Pharmacology Research: It is widely used in early drug development processes such as disease modeling, target identification and verification, drug screening, and efficacy and safety evaluation.

·?Stem Cell Research: It can significantly improve the survival rate and regenerative capacity of human embryonic stem cell-derived pluripotent stem cells and adult somatic stem cells after in vivo implantation, fully unleashing the potential of stem cells in the fields of disease modeling and regenerative medicine.

When it comes to high-quality collagen products, Advanced BioMatrix (referred to as?ABM?for short) is a name that cannot be omitted. This well-known U.S. company, with 25 years of experience in R&D and manufacturing, focuses on providing high-quality products for research fields such as tissue engineering, cell analysis, and cell proliferation. ABM has established a high-standard production and testing platform for collagen products, and each of its three best-selling Type I Collagen products is a "powerhouse" in terms of performance.

1. VitroCol? HUMAN COLLAGEN SOLUTION, Type I (Catalog No.: 5007 - 20 ml)

It is derived from the secretion of human fibroblasts, with a purity of up to 97%. Not only does it pass sterility testing, but it also has a concentration of 3 mg/ml. It is suitable for coating cell culture dishes—especially effective for culturing cells that are difficult to adhere to ordinary culture vessels—and can also be used for preparing 3D collagen gels.

2. RatCol? Rat Tail Type I Collagen with Neutralization Solution for 3D Gels (Catalog No.: 5153 - 100 mg)

Extracted from rat tail tendons, it has a purity of 95%, tests negative for sterility, and has a concentration of 4 mg/ml. It is the ideal choice for preparing 3D collagen gels.

3. RatCol Rat Tail Type I Collagen for Coatings (Catalog No.: 5006 - 20 ml)

Also derived from rat tail tendons, it has a purity of 95% and tests negative for sterility. Its concentration of 4 mg/ml makes it an ideal option for coating cell culture vessels.